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Ligand-receptor capture with living cell, LRC-TriCEPS by Dualsystems Biotech AG

Protein-protein interaction on the cell membrane of the living cell

Ligand-receptor capture with living cell, LRC-TriCEPS by Dualsystems Biotech AG

Proteomics expert for protein protein interaction


Dualsystems Biotech AG is specialized in elucidating protein-protein interactions in the living cells especially at the cell membrane. Dualsystems is a privately held service provider company based in Schlieren (Zurich), Switzerland. Our clients are from big pharma, biotech and academia. We focus on our conceptually new technology for target deconvolution the Ligand-Receptor Capture LRC-TriCEPS™ platform. The LRC-TriCEPS™ technology is able to identify the unknown targets, off-targets, receptors of your orphan ligand on the living cells and is an excellent tool to investigate the binding partners of your drug candidates. Following orphan ligands can be used: peptides, proteins, antibodies, viruses.

LRC-TriCEPS™ – Ligand-receptor capture Service


Identification of the targets and off targets at the cell surface on the living cells


Ligand-receptor capture technology LRC-TriCEPS™

A novel LC-MS/MS based approach to identify the cell surface receptors and off-targets for a ligand of interest after protein-protein interaction on the living cell. The LRC-TriCEPS technology was invented by Dr. Andreas Frei and Prof. Bernd Wollscheid from the ETH Zurich who published the technology in Nature Biotechnology (Frei et al.(2012) Nature Biotechnology 30:997-1001).

Now a new Version of TriCEPS is available. For more details click here Details

Orphan ligands:
Receptor-mit-Ligand-Ausschnitt-2016-small

  • Extracellular proteins
  • Peptide ligands
  • Antibodies
  • Viruses
  • Engineered affinity binders

LRC-TriCEPS™ Services (target deconvolution on the living cells)

To see how we can support you in your project please contact us with the form below or just give us a call .

Flow TriCEPS™ Services/Kit


Cell selection and optimization of binding conditions for target identification


Identification of the targets using TriCEPS V2.0 in or at the cell membrane of your ligand (peptide, protein, Antibody, ADC or other primary amine containing molecules) requires that the cells used are expressing the unknown targets. TriCEPS V2.0 is the ideal tool to screen cell lines or primary cells to identify the best cells to use later in the LRC-TriCEPS target identification experiments. Dualsystems offers these pretests for cell selection as service and kit. Needed for the experiment is the ligand of interest and the cell lines/ primary cells that are expected to express the unknown target. There is no need for a ligand specific antibody.

Flow (Pretest) TriCEPS™ Service and Kit is available. For more details click here Details

Used for:

  • Screening of different cells
  • Cell selection
  • Positive control selection
  • Negative control selection
  • Functional Assays with TriCEPS coupled ligand
  • Flow Cytometric (FACS) reagent to analyze protein/peptide binding to cells